Why buffer is important

Jun 22 Nov 24 May 04 Nov 13 May 10 Feb 19 Jan 15 Oct 25 Aug 24 Control over the variables in an experiment or analysis is an important element to make sure the analysis is accurate and repeatable.

There are many variables that you might have to consider, both chemical and physical, during the analysis. For example, a variable like temperature can be controlled using an oven or water bath. When adding small amounts of a sample to a solvent, you can cause chemical changes such as a change in pH. This can lead to further changes such as changes in solubility and sample precipitation. Whilst this can be annoying in a beaker — imagine if it happens in a chromatography column.

So how can we control a variable like pH? So in HPLC for example, if adding a sample to a mobile phase can shift the pH, a buffer can be added to the mobile phase to resist the pH changes — giving the analyst control over the pH. A buffer solution has to resist these changes. Under conditions when excess hydrogen enters the cell, it reacts with the hydrogen phosphate ions, which accepts them.

Under alkaline conditions, the dihydrogen phosphate ions accept the excess hydroxide ions that enter the cell. Proteins consist of amino acids held together by peptide bonds. The amino acids possess an amino group and a carboxylic acid group. When the pH becomes acidic, the carboxyl group takes up excess hydrogen ions to return back to the carboxylic acid form. The respiratory pigment present in blood, hemoglobin, also has buffering action within tissues.

It has an ability to bind with either protons or oxygen at a given point of time. Binding of one releases the other. In hemoglobin, the binding of protons occurs in the globin portion whereas oxygen binding occurs at the iron of the heme portion. At the time of exercise, protons are generated in excess. Hemoglobin helps in the buffering action by binding these protons, and simultaneously releasing molecular oxygen. In computer science, a data buffer or just buffer is a region of a physical memory storage used to temporarily store data while it is being moved from one place to another.

However, a buffer may be used when moving data between processes within a computer. The buffer health tells you how much video buffer it currently has downloaded waiting to be played.

If you have plenty of available bandwidth, it can get away with using a smaller buffer since it can quickly download more as needed. Buffer days feel unrewarding at the time but make a big difference. Buffer days are what make focus days possible. Buffer zones are areas created to enhance the protection of a specific conservation area, often peripheral to it. Within buffer zones, resource use may be legally or customarily restricted, often to a lesser degree than in the adjacent protected area so as to form a transition zone.

In this buffer, hydronium and bicarbonate anion are in equilibrium with carbonic acid. In this page you can discover 37 synonyms, antonyms, idiomatic expressions, and related words for buffer, like: shock-absorber, backstop, buffer storage, bumper, safeguard, cushion, shield, barrier, defense, fender and pad. A safeguard is a law, rule, or measure intended to prevent someone or something from being harmed. As an additional safeguard against weeds you can always use an underlay of heavy duty polyethylene.

Synonyms: protection, security, defence, guard More Synonyms of safeguard. The three major buffer systems of our body are carbonic acid bicarbonate buffer system, phosphate buffer system and protein buffer system. In the human stomach and duodenum, the bicarbonate buffer system serves to both neutralize gastric acid and stabilize the intracellular pH of epithelial cells via the secretion of bicarbonate ion into the gastric mucosa.

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